Certified Hemodialysis Technician Practice Exam 2026 – All-in-One Guide to Exam Success

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What medium is used for testing refrigerated water and dialysate samples?

Nutrient Agar

Tryptic Soy Agar

In the context of testing refrigerated water and dialysate samples, Tryptic Soy Agar is a preferred medium due to its broad-spectrum nutrient profile that supports the growth of a wide variety of bacteria. This is particularly important in dialysis settings, where the presence of microbial contamination can pose serious health risks to patients. Tryptic Soy Agar contains nutrients derived from soybean meal and casein, which provide the essential amino acids and other growth factors necessary for the cultivation of many types of bacteria.

Using this medium allows technicians to effectively assess the microbial quality of these samples by incubating them and observing for growth. The versatility of Tryptic Soy Agar makes it suitable for detecting environmental and opportunistic pathogens that may thrive in dialysate or water systems.

Other mediums, while useful for specific types of testing, do not offer the same level of general applicability to the variety of organisms that might contaminate water or dialysate. For instance, Nutrient Agar is geared more towards general bacterial growth without some of the broader applications, Blood Agar is specialized for the growth of fastidious organisms and may not be as efficient for standard water quality assessments, and Nutrient Broth does not provide the solid surface necessary for colony counting or differentiation as effectively as agar

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Blood Agar

Nutrient Broth

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